INNOVATION CENTER FOR GENE MEDICINE

충남대학교 신약전문대학원 & 대전테크노파크 바이오센터

기술상담

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Typical Workflow for Viral Gene Delivery
  • Cloning, Gene
    of interest
  • Packaging virus
  • Concentration
    & Purification
  • Titration
  • Delivery into
    cells or animal
Comparison of Viral Vectors for Gene Delivery
  Adeno-associated Virus (AAV) Lentivirus
Genome ssDNA ssRNA (+)
(reverstranscription)
Coat Non-enveloped Enveloped
Genome size (virus size) 5kb (18~26nm) 9 kb (80~130nm)
Packaging Capacity ~4.5 kb < 8 kb
Infection/tropism Dividing and non-dividing cells Dividing and non-dividing cells
Transgene expression Potentially long-lasting long-lasting
Relative Foreign Gene Expression Medium Medium
Immune Response Very Low Low
Integrates into target cell genome Non-integrating** :Native AAV can integrate,
but recombinant AAV rarely does.
Integrating or non-integrating
Relative transduction efficiency +++ +++
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